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immune-magnetic selection method manual easysep® protocol using purple easysep® magnet  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc immune-magnetic selection method manual easysep® protocol using purple easysep® magnet
    Immune Magnetic Selection Method Manual Easysep® Protocol Using Purple Easysep® Magnet, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immune-magnetic selection method manual easysep® protocol using purple easysep® magnet/product/STEMCELL Technologies Inc
    Average 90 stars, based on 1 article reviews
    immune-magnetic selection method manual easysep® protocol using purple easysep® magnet - by Bioz Stars, 2026-03
    90/100 stars

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    Phenotype comparison of adhered and suspended cell immature DCs population derived from flask culture method and EasySep Magnet Positive Selection kit based method.

    Journal: Bone Marrow Research

    Article Title: Optimization of Ex Vivo Murine Bone Marrow Derived Immature Dendritic Cells: A Comparative Analysis of Flask Culture Method and Mouse CD11c Positive Selection Kit Method

    doi: 10.1155/2018/3495086

    Figure Lengend Snippet: Phenotype comparison of adhered and suspended cell immature DCs population derived from flask culture method and EasySep Magnet Positive Selection kit based method.

    Article Snippet: In our study, the effects of two CD11c DC separation methods including flask culture method and EasySep kit method (commercially available Kit-Stem Cell Technologies) were compared regarding the production of immature BMDCs.

    Techniques: Comparison, Derivative Assay, Selection, Expressing

    Representative histogram plot ( n = 3) for CD11c+ expression on BMDCs. Bone marrow cells were cultured in RPMI 1640 medium with 10% FBS containing low doses of rmGM-CSF (as described in Materials and Methods) and harvested at day 12 for flask culture method and day 5 for EasySep Magnet Positive Selection kit based method. Untreated (sky blue) and low doses of rmGM-CSF treated (pink) BMDCs were stained with PE-labeled anti-CD11c and analyzed by flow cytometry. DCs were gated based on CD11c+ staining. Data were analyzed using Flowjo, LLC, Oregon, USA, software. Images show purity of CD11c+ cells with regard to percentages of adhered CD11c count generated by (a) flask culture method and (c) by EasySep Magnet Positive Selection kit based method and percentages of suspended cell population by (b) flask culture method and (d) by EasySep Magnet Positive Selection kit based method are indicated in the respective histograms ( n = 3).

    Journal: Bone Marrow Research

    Article Title: Optimization of Ex Vivo Murine Bone Marrow Derived Immature Dendritic Cells: A Comparative Analysis of Flask Culture Method and Mouse CD11c Positive Selection Kit Method

    doi: 10.1155/2018/3495086

    Figure Lengend Snippet: Representative histogram plot ( n = 3) for CD11c+ expression on BMDCs. Bone marrow cells were cultured in RPMI 1640 medium with 10% FBS containing low doses of rmGM-CSF (as described in Materials and Methods) and harvested at day 12 for flask culture method and day 5 for EasySep Magnet Positive Selection kit based method. Untreated (sky blue) and low doses of rmGM-CSF treated (pink) BMDCs were stained with PE-labeled anti-CD11c and analyzed by flow cytometry. DCs were gated based on CD11c+ staining. Data were analyzed using Flowjo, LLC, Oregon, USA, software. Images show purity of CD11c+ cells with regard to percentages of adhered CD11c count generated by (a) flask culture method and (c) by EasySep Magnet Positive Selection kit based method and percentages of suspended cell population by (b) flask culture method and (d) by EasySep Magnet Positive Selection kit based method are indicated in the respective histograms ( n = 3).

    Article Snippet: In our study, the effects of two CD11c DC separation methods including flask culture method and EasySep kit method (commercially available Kit-Stem Cell Technologies) were compared regarding the production of immature BMDCs.

    Techniques: Expressing, Cell Culture, Selection, Staining, Labeling, Flow Cytometry, Software, Generated